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Western blot analysis of FAAH2 Antibody in HepG2 cell line lysates (35ug/lane)
FAAH2 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of FAAH2 Antibody in HepG2 cell line lysates (35ug/lane)

FAAH2 Antibody

CATALOG NUMBER: 61-810

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FAAH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-438 amino acids from the C-terminal region of human FAAH2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FAAH2
Additional Names:
Fatty-acid amide hydrolase 2, Amidase domain-containing protein, Anandamide amidohydrolase 2, Oleamide hydrolase 2, FAAH2, AMDD
Protein Accession Number:
Q6GMR7
Protein Gi Number:
74757585
Ncbi Gene Id Number:
158584
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FAAH2 encodes a fatty acid amide hydrolase that shares a conserved protein motif with the amidase signature family of enzymes. The encoded enzyme is able to catalyze the hydrolysis of a broad range of bioactive lipids, including those from the three main classes of fatty acid amides; N-acylethanolamines, fatty acid primary amides and N-acyl amino acids. This enzyme has a preference for monounsaturated acyl chains as a substrate.
Background Reference 1:
Kaczocha, M., et al. J. Biol. Chem. 285(4):2796-2806(2010)
Background Reference 2:
Karbarz, M.J., et al. Anesth. Analg. 108(1):316-329(2009)
Background Reference 3:
Wei, B.Q., et al. J. Biol. Chem. 281(48):36569-36578(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-810

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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