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HEK293 overexpressing FABP2 and probed with 45-561 (mock transfection in first lane).
45-561 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-561 (0.001ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
HEK293 overexpressing FABP2 and probed with 45-561 (mock transfection in first lane).

FABP2 Antibody

CATALOG NUMBER: 45-561

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EGVEAKRIFKKD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:A very strong approx 16kDa band observed in Human Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.2). Primary incubation was 1 hour. In transfected HEK293 transiently expressing FABP2 a band of approx 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.001ug/ml.Immunohistochemistry:In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration: 2-3ug/ml.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FABP2
Additional Names:
FABP2, fatty acid binding protein 2, intestinal , FABPI, I-FABP, MGC133132 , Fatty acid-binding protein, intestinal, intestinal fatty acid binding protein 2
Protein Accession Number:
NP_000125.2
Protein Gi Number:
4826720
Ncbi Gene Id Number:
2169
Background Reference 1:
Takakura Y, Yoshioka K, Umekawa T, Kogure A, Toda H, Yoshikawa T, Yoshida T. Thr54 allele of the FABP2 gene affects resting metabolic rate and visceral obesity. Diabetes Res Clin Pract. 2005 Jan;67(1):36-42.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-561

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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