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Western blot analysis of extracts of various cell lines, using FABP4 antibody (13-092).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat heart using FABP4 antibody (13-092) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using FABP4 antibody (13-092) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FABP4 antibody (13-092) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using FABP4 antibody (13-092). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using FABP4 antibody (13-092).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

FABP4 Antibody

CATALOG NUMBER: 13-092

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human FABP4 (NP_001433.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse heart
Positive Control 2:
Mouse trachea
Predicted Molecular Weight:
Observed: 17kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FABP4
Additional Names:
Fatty Acid Binding Protein 4; Adipocyte Fatty Acid Binding Protein; ALBP; Adipocyte Lipid Binding Protein
Ncbi Gene Id Number:
2167
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-092

List Size:
100 uL

List Price:

$436.00

Datasheet
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