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IHC testing of FFPE human placenta with Factor XIIIa antibody (clone SPM180). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human placenta with Factor XIIIa antibody (clone SPM180). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Factor XIIIa Antibody [SPM180]

CATALOG NUMBER: 33-778

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A human partial recombinant protein corresponding to amino acids 46-181 was used as the immunogen for the Factor XIIIa antibody.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC-P, WB
Application Note:
ELISA: 2-4 ug/ml; order the BSA-free formulation for coating
IF: 1-2 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
WB: 0.5-1 ug/ml
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Titering of the Factor XIIIa antibody may be required for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
F13A1
Additional Names:
F13A1, coagulation factor XIII, A1 polypeptide, RP11-232H4.1, F13A, Coagulation factor XIII A chain, Coagulation factor XIII, A polypeptide, TGase, bA525O21.1 (coagulation factor XIII, A1 polypeptide), coagulation factor XIII A1 subunit, factor XIIIa
Ncbi Gene Id Number:
2162
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Factor XIIIa has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-Factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-778

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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