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Fluorescent image of A549 cell stained with FADS2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.
Western blot analysis in HepG2 cell line lysates (35ug/lane).
FADS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent image of A549 cell stained with FADS2 Antibody (N-term). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FADS2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).FADS2 immunoreactivity is localized to Cytoplasm and Vesicles significantly.

FADS2 Antibody

CATALOG NUMBER: 56-329

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This FADS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human FADS2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FADS2
Additional Names:
Fatty acid desaturase 2, 11419-, Delta(6) fatty acid desaturase, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2
Protein Accession Number:
O95864
Ncbi Gene Id Number:
9415
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the fattyacid desaturase (FADS) gene family. Desaturase enzymes regulateunsaturation of fatty acids through the introduction of doublebonds between defined carbons of the fatty acyl chain. FADS familymembers are considered fusion products composed of an N-terminalcytochrome b5-like domain and a C-terminal multiplemembrane-spanning desaturase portion, both of which arecharacterized by conserved histidine motifs. This gene is clusteredwith family members FADS1 and FADS2 at 11q12-q13.1; this cluster isthought to have arisen evolutionarily from gene duplication basedon its similar exon/intron organization.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Mathias, R.A., et al. J. Lipid Res. 51(9):2766-2774(2010)
Background Reference 3:
Lu, Y., et al. Am. J. Clin. Nutr. 92(1):258-265(2010)
Background Reference 4:
Zabaneh, D., et al. PLoS ONE(8), E11961 (2010) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-329

List Size:
200 ul

List Price:

$495.00

Datasheet
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