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Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577).
Immunofluorescence staining of methanol-fixed HeLa cells using FAK (phospho-Tyr576/Tyr577).
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577).

FAK (phospho Tyr576 / Tyr577) Antibody

CATALOG NUMBER: 79-511

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
FAK (phospho-Tyr576/Tyr577) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y (p) -Y (p) -K-A) derived from Human FAK.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577.
Predicted Molecular Weight:
125 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
PTK2
Additional Names:
FAK, FADK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, EIF4F, EIF4G, EIF4GI, FADK 1, PTK2
Protein Accession Number:
NP_005598.3
Protein Gi Number:
27886593
Ncbi Gene Id Number:
5747
Background:
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Background Reference 1:
Parsons, J. T. et al. (2000) Oncogene 19, 5606-5613Schaller, M. D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688. Cobb, B. S. et al. (1994) Mol. Cell. Biol. 14, 147-155. Chen, H. C. et al. (1996) J. Biol. Chem. 271, 26329-26334.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-511

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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