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Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.

FAK1 Antibody

CATALOG NUMBER: 63-158

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat, Xenopus
Immunogen:
This FAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 557-587 amino acids from human FAK1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
119 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PTK2
Additional Names:
Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regulatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, PTK2, FAK, FAK1
Protein Accession Number:
Q05397
Protein Gi Number:
3183518
Ncbi Gene Id Number:
5747
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FAK1 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of the gene encoding FAK1 may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix.
Background Reference 1:
Okamoto, H., et al., Hepatology 38(5):1242-1249 (2003).
Background Reference 2:
Moritake, H., et al., Cancer Genet. Cytogenet. 146(2):102-109 (2003).
Background Reference 3:
Liu, X.W., et al., J. Biol. Chem. 278(41):40364-40372 (2003).
Background Reference 4:
Avraham, H.K., et al., J. Biol. Chem. 278(38):36661-36668 (2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-158

List Size:
400 ul

List Price:

$495.00

Datasheet
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