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Western blot analysis of extracts of various cell lines, using FAM20A antibody (23-624) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using FAM20A antibody (23-624) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using FAM20A antibody (23-624) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using FAM20A antibody (23-624) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using FAM20A antibody (23-624) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

FAM20A Antibody

CATALOG NUMBER: 23-624

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 140-240 of human FAM20A (NP_060035.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
Positive Control 1:
SH-SY5Y
Positive Control 2:
Mouse small intestine
Positive Control 3:
Mouse brain
Positive Control 4:
Rat liver
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FAM20A
Additional Names:
Protein FAM20A, FAM20A
Ncbi Gene Id Number:
54757
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This locus encodes a protein that is likely secreted and may function in hematopoiesis. A mutation at this locus has been associated with amelogenesis imperfecta and gingival hyperplasia syndrome. Alternatively spliced transcript variants have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-624

List Size:
100 uL

List Price:

$436.00

Datasheet
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