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Fluorescent image of Hela cells stained with FAM50A Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis in A549,Hela cell line lysates (35ug/lane).
Fluorescent image of Hela cells stained with FAM50A Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

FAM50A Antibody

CATALOG NUMBER: 60-987

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Zebrafish, Mouse
Immunogen:
This FAM50A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-339 amino acids from the C-terminal region of human FAM50A.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For IF starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FAM50A
Additional Names:
Protein FAM50A, Protein HXC-26, Protein XAP-5, FAM50A, DXS9928E, HXC26, XAP5
Protein Accession Number:
Q14320
Protein Gi Number:
12231058
Ncbi Gene Id Number:
9130
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
May be a DNA-binding protein or transcriptional factor.
Background Reference 1:
Mazzarella R., et al. Genomics 45:216-219(1997).
Background Reference 2:
Ota T., et al. Nat. Genet. 36:40-45(2004).
Background Reference 3:
Ross M.T., et al. Nature 434:325-337(2005).
Background Reference 4:
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-987

List Size:
400 ul

List Price:

$495.00

Datasheet
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