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Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.
Immunofluorescence of FAM59A in Human Liver cells with FAM59A antibody at 20 μg/mL.
Western blot analysis of FAM59A in rat liver tissue lysate with FAM59A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

FAM59A Antibody

CATALOG NUMBER: 5479

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A.
The immunogen is located within amino acids 630 - 680 of FAM59A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
FAM59A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FAM59A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FAM59A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FAM59A
Additional Names:
FAM59A Antibody: Gm944, FAM59A, C18orf11, GRB2-associated and regulator of MAPK protein, GRB2-associated and regulator of MAPK1
Protein Accession Number:
Q9H706
Protein Gi Number:
125991851
Ncbi Gene Id Number:
64762
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FAM59A Antibody: FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.
Background Reference 1:
Blagoev B, Ong SE, Kratchmarova I, et al. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat. Biotechnol.2004; 22:1139-45.
Background Reference 2:
Tashiro K, Tsunematsu T, Okubo H, et al. GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through activation of extracellular signal-regulated kinase signaling. J. Biol. Chem.2009; 284:20206-14.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5479

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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