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46-657 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing FAM62A and probed with 46-657 (mock transfection in first lane).
46-657 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-657 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

FAM62A Antibody

CATALOG NUMBER: 46-657

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QKKRTLSPEFNER
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 150kDa band observed in Human Adipose lysates (calculated MW of 123kDa according to NP_056107.1). In transfected HEK293 transiently expressing FAM62A a band of approx. 120kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. XBL-11048 - Human Adipose Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FAM62A
Additional Names:
FAM62A, family with sequence similarity 62 (C2 domain containing), member A, KIAA0747, MBC2, membrane bound C2 domain containing protein, vp115
Protein Accession Number:
NP_056107.1
Protein Gi Number:
14149680
Ncbi Gene Id Number:
23344
Background Reference 1:
Morris NJ, Ross SA, Neveu JM, Lane WS, Lienhard GE. Cloning and preliminary characterization of a 121 kDa protein with multiple predicted C2 domains. Biochim Biophys Acta. 1999 May 18;1431(2):525-30.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-657

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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