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Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody .AP9522b was diluted at 1:1000 at each lane.
Immunofluorescent analysis of U251 cells, using FANCC Antibody . Antibody was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
FANCC Antibody IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody .AP9522b was diluted at 1:1000 at each lane.

FANCC Antibody

CATALOG NUMBER: 63-986

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FANCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-555 amino acids from the C-terminal region of human FANCC.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:25
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FANCC
Additional Names:
Fanconi anemia group C protein, Protein FACC, FANCC, FAC, FACC
Protein Accession Number:
Q00597
Protein Gi Number:
1706762
Ncbi Gene Id Number:
2176
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This protein is for complementation group C.
Background Reference 1:
Barroso, E., et al. Breast Cancer Res. Treat. 118(3):655-660(2009)
Background Reference 2:
McWilliams, R.R., et al. Cancer Epidemiol. Biomarkers Prev. 18(9):2549-2552(2009)
Background Reference 3:
Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
Background Reference 4:
Palmieri, R.T., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3567-3572(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-986

List Size:
400 ul

List Price:

$495.00

Datasheet
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