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45-567 (0.5ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing FANCG and probed with 45-567 (mock transfection in first lane).
45-567 (1ug/ml) staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-567 (3ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-567 (3ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-567 (0.5ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

FANCG Antibody

CATALOG NUMBER: 45-567

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: LEEFRTSLPKSCDL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa, and in nuclear lysates of cell line Jurkat (calculated MW of 68.6kDa according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Uterus and Spleen. Recommended concentration: 3ug/ml.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FANCG
Additional Names:
FANCG, XRCC9, Fanconi anemia, complementation group G, FAG, DNA repair protein XRCC9, X-ray repair, complementing defective, in Chinese hamster, 9, X-ray repair complementing defective repair in Chinese hamster cells 9
Protein Accession Number:
NP_004620.1
Protein Gi Number:
4759336
Ncbi Gene Id Number:
2189
Background Reference 1:
Liu N, Lamerdin JE, Tucker JD, Zhou ZQ, Walter CA, Albala JS, Busch DB, Thompson LH. The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells. Proc Natl Acad Sci USA. 1997 Aug 19;94(17):9232-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-567

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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