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Western blot analysis of anti-FARSA Pab in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FARSA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-FARSA Pab in K562 cell line lysates (35ug/lane)

FARSA Antibody

CATALOG NUMBER: 62-996

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This FARSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human FARSA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FARSA
Additional Names:
Phenylalanine--tRNA ligase alpha subunit, CML33, Phenylalanyl-tRNA synthetase alpha subunit, PheRS, FARSA, FARS, FARSL, FARSLA
Protein Accession Number:
Q9Y285
Protein Gi Number:
12643946
Ncbi Gene Id Number:
2193
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARSA is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This protein has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression.
Background Reference 1:
Gevaert,K., Nat. Biotechnol. 21 (5), 566-569 (2003)
Background Reference 2:
Moor,N.,Protein Expr. Purif. 24 (2), 260-267 (2002)
Background Reference 3:
Sen,S., Proc. Natl. Acad. Sci. U.S.A. 94 (12), 6164-6169 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-996

List Size:
400 ul

List Price:

$495.00

Datasheet
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