Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<b> Flow-cytometry using the anti-Fas antibody R-125224 (</b> Jurkat cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control- black line) or the r-chimeric version of R-125224 (24-527- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Western Blot using anti-Fas antibody R-125224 (</b> Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric r version of R-125224 (24-527) at 2 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa- but this protein is glycosylated at several positions leading to the observed running size.
<b> Immunofluorescence staining of fixed MCF7 cells with anti-Fas antibody R-125224 (</b> Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of R-125224 (24-527) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-527- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-Fas antibody R-125224 ( Jurkat cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control- black line) or the r-chimeric version of R-125224 (24-527- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Fas Antibody [R-125224], Rabbit IgG

CATALOG NUMBER: 24-527

Clonality:
Recombinant Monoclonal
Tested Applications:
Activation, ELISA, Flow, Imaging
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Human
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
R-125224 is generated by the humanization of the murine HFE7A anti-Fas antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas or the L5178YA1 cell line expressing murine Fas, but not to the parental WR19L or L5178Y cells.
Conjugate:
Unconjugated
Tested Applications:
Activation, ELISA, Flow, Imaging
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
FAS
Additional Names:
CD95, h-HFE7A, Tumor necrosis factor receptor superfamily member 6, CD-95, Apo-1, Apo 1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor
Ncbi Gene Id Number:
355
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-527

List Size:
0.2 mg

List Price:

$598.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369