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Western blot analysis of Fascin Antibody in Y79 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma with Fascin Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using Fascin Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of Fascin Antibody in Y79 cell line lysates (35ug/lane)

Fascin Antibody

CATALOG NUMBER: 62-436

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Fascin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the Central region of human Fascin.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FSCN1
Additional Names:
Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55, FSCN1, FAN1, HSN, SNL
Protein Accession Number:
Q16658
Protein Gi Number:
2498357
Ncbi Gene Id Number:
6624
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. It is probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.
Background Reference 1:
Qualtrough,D., Br. J. Cancer 101 (7), 1124-1129 (2009)
Background Reference 2:
Ono,S., J. Biol. Chem. 272 (4), 2527-2533 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-436

List Size:
400 ul

List Price:

$495.00

Datasheet
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