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Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.
Western blot analysis of Fat Free in mouse brain tissue lysate with Fat Free antibody at 1 μg/mL.
Trial Size

Fat Free Antibody

CATALOG NUMBER: 6979

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (82%)
Immunogen:
Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free.
The immunogen is located within amino acids 620 - 670 of Fat Free.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
86 kDa
Purification:
Fat Free Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Fat Free antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
C11orf2
Additional Names:
Fat Free Antibody: FFR, ANG2, ANG3, C11orf2, C11orf3, FFR, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein
Protein Accession Number:
NP_037397
Protein Gi Number:
8393009
Ncbi Gene Id Number:
738
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.
Background Reference 1:
Perez-Victoria FJ, Schindler C, Magadan JG, et al. Ang2/Fat Free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol. Biol. Cell 2010; 21:3386-95
Background Reference 2:
Ho SY, Lorent K, Pack M, et al. Zebrafish Fat Free is required for intestinal lipid absorption and Golgi apparatus structure. Cell Metab. 2006; 3:289-300.
Background Reference 3:
Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell 2008; 19:2350-62
Background Reference 4:
Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6979

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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