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45-571 (0.3ug/ml) staining of Human Frontal Cortex (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
45-571 (10ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-571 (0.3ug/ml) staining of Human Frontal Cortex (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence

FBL2 Antibody

CATALOG NUMBER: 45-571

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: VFSNNDEGLINKK-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Brain (Frontal Cortex) lysates and approx 50kDa band observed in Rat Brain lysates (calculated MW of 47.1kDa according to Human NP_036289.2, and 46.9kDa according to Rat XP_343496.4). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in neuronal bodies. Recommended concentration: 5-10ug/ml.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FBXL2
Additional Names:
FBXL2, FBL2, F-box and leucine-rich repeat protein 2, FBL3, DKFZP564P0622, F-box protein containing leucine-rich repeats
Protein Accession Number:
NP_036289.2
Protein Gi Number:
16306582
Ncbi Gene Id Number:
25827
Background Reference 1:
Ilyin GP, Rialland M, Pigeon C, Guguen-Guillouzo C. cDNA cloning and expression analysis of new members of the mammalian F-box protein family. Genomics. 2000 Jul 1;67(1):40-7.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-571

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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