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Western blot analysis in A549 cell line lysates (35ug/lane).
FBLIM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in A549 cell line lysates (35ug/lane).

FBLIM1 Antibody

CATALOG NUMBER: 56-601

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FBLIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the C-terminal region of human FBLIM1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBLIM1
Additional Names:
Filamin-binding LIM protein 1, FBLP-1, Migfilin, Mitogen-inducible 2-interacting protein, MIG2-interacting protein, FBLIM1, FBLP1
Protein Accession Number:
Q8WUP2
Protein Gi Number:
125987829
Ncbi Gene Id Number:
54751
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein with an N-terminalfilamin-binding domain, a central proline-rich domain, and,multiple C-terminal LIM domains. This protein localizes at celljunctions and may link cell adhesion structures to the actincytoskeleton. This protein may be involved in the assembly andstabilization of actin-filaments and likely plays a role inmodulating cell adhesion, cell morphology and cell motility. Thisprotein also localizes to the nucleus and may affect cardiomyocytedifferentiation after binding with the CSX/NKX2-5 transcriptionfactor. Alternative splicing results in multiple transcriptvariants encoding different isoforms.
Background Reference 1:
Zhao, J., et al. J. Biol. Chem. 284(49):34308-34320(2009)
Background Reference 2:
Ithychanda, S.S., et al. J. Biol. Chem. 284(7):4713-4722(2009)
Background Reference 3:
Lad, Y., et al. J. Biol. Chem. 283(50):35154-35163(2008)
Background Reference 4:
Lai-Cheong, J.E., et al. J. Invest. Dermatol. 128(9):2156-2165(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-601

List Size:
400 ul

List Price:

$495.00

Datasheet
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