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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).  This demonstrates the FBXL14 antibody detected the FBXL14 protein (arrow).
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).  This demonstrates the FBXL14 antibody detected the FBXL14 protein (arrow).

FBXL14 Antibody

CATALOG NUMBER: 61-838

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FBXL14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-407 amino acids from the C-terminal region of human FBXL14.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXL14
Additional Names:
F-box/LRR-repeat protein 14, F-box and leucine-rich repeat protein 14, FBXL14, FBL14
Protein Accession Number:
Q8N1E6
Protein Gi Number:
48428083
Ncbi Gene Id Number:
144699
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FBXL14 is members of the F-box protein family, such as FBXL14, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.
Background Reference 1:
Vinas-Castells, R., et al. J. Biol. Chem. 285(6):3794-3805(2010)
Background Reference 2:
Jin, J., et al. Genes Dev. 18(21):2573-2580(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-838

List Size:
400 ul

List Price:

$495.00

Datasheet
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