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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

FBXL16 Antibody

CATALOG NUMBER: 31-301

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXL16.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
FBXL16 antibody can be used for detection of FBXL16 by ELISA at 1:62500. FBXL16 antibody can be used for detection of FBXL16 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store FBXL16 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
FBXL16
Additional Names:
FBXL16, C16orf22, FLJ33735, Fbl16, MGC33974, c380A1.1
Protein Accession Number:
NP_699181
Protein Gi Number:
289666746
Ncbi Gene Id Number:
146330
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FBXL16 is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.Members of the F-box protein family, such as FBXL16, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).
Background Reference 1:
Martin, J., (2004) Nature 432 (7020), 988-994 .

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

31-301

List Size:
100 ul

List Price:

$519.00

Datasheet
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