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Western blot analysis of FBXL16 in human spleen tissue lysate with FBXL16 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of FBXL16 in human spleen tissue with FBXL16 antibody at 5 μg/mL.
Immunofluorescence of FBXL16 in human spleen tissue with FBXL16 antibody at 20 μg/mL.
Western blot analysis of FBXL16 in human spleen tissue lysate with FBXL16 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

FBXL16 Antibody

CATALOG NUMBER: 6165

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
FBXL16 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human FBXL16.
The immunogen is located within the last 50 amino acids of FBXL16.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
FBXL16 antibody can be used for detection of FBXL16 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
FBXL16 antibody is predicted to not cross-react with other F-box protein family members.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
FBXL16 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FBXL16 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FBXL16 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXL16
Additional Names:
FBXL16 Antibody: Fbl16, C16orf22, c380A1.1, FBL16, F-box and leucine-rich repeat protein 16
Protein Accession Number:
NP_699181
Protein Gi Number:
289666746
Ncbi Gene Id Number:
146330
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FBXL16 Antibody: FBXL16 (F-box and leucine-rich repeat protein 16), a member of the F-box protein family, is a probable substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. It is characterized by an approximately 40-amino acid F-box motif. F-box proteins interact with SKP1 through the F-box, and they interact with ubiquitination targets through other protein interaction domains. FBXL16 is a novel E2F1-regulated gene in crucial cell cycle regulation pathways.
Background Reference 1:
Martin J, Han C, Gordon LA, et al. The sequence and analysis of duplication-rich human chromosome 16. Nature2004; 432:988-94.
Background Reference 2:
Jin J, Cardozo T, Lovering RC, et al. Systematic analysis and nomenclature of mammalian F-box proteins. Genes Dev.2004; 18:2573-80.
Background Reference 3:
Sato K, Kusama Y, Tategu M, et al. FBXL16 is a novel E2F1-regulated gene commonly upregulated in p16INK4A- and p14ARF-silenced HeLa cells. Int. J. Oncol.2010; 36:479-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6165

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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