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Western blot analysis of FBXL2 Antibody in MDA-MB435 cell line lysates (35ug/lane)
FBXL2 Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of FBXL2 Antibody in MDA-MB435 cell line lysates (35ug/lane)

FBXL2 Antibody

CATALOG NUMBER: 61-762

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This FBXL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-395 amino acids from the C-terminal region of human FBXL2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXL2
Additional Names:
F-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2 {ECO:0000312, HGNC:HGNC:13598}, F-box protein FBL2/FBL3 {ECO:0000303, PubMed:10945468, ECO:0000312, EMBL:AAF045101}, FBXL2 (HGNC:13598)
Protein Accession Number:
Q9UKC9
Protein Gi Number:
145559475
Ncbi Gene Id Number:
25827
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FBXL2 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats.
Background Reference 1:
Ye, J. PLoS Pathog.(8), E108 (2007)
Background Reference 2:
Ilyin, G.P., et al. Genomics 67(1):40-47(2000)
Background Reference 3:
Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-762

List Size:
400 ul

List Price:

$495.00

Datasheet
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