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Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.

FBXO21 Antibody

CATALOG NUMBER: 25-830

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXO21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
FBXO21 antibody can be used for detection of FBXO21 by ELISA at 1:312500. FBXO21 antibody can be used for detection of FBXO21 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
293T Cell Lysate
Predicted Molecular Weight:
71 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store FBXO21 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
FBXO21
Additional Names:
FBXO21, DKFZp434G058, FBX21, FLJ90233, KIAA0875, MGC26682
Protein Accession Number:
NP_055817
Protein Gi Number:
16306500
Ncbi Gene Id Number:
23014
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FBXO21 contains 1 F-box domain. It is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.
Background Reference 1:
Nakayama, M., (2002) Genome Res. 12 (11), 1773-1784.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-830

List Size:
100 ul

List Price:

$519.00

Datasheet
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