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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis in mouse kidney tissue lysates (35ug/lane).
FBXO24 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

FBXO24 Antibody

CATALOG NUMBER: 55-556

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This FBXO24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human FBXO24.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXO24
Additional Names:
F-box only protein 24, FBXO24, FBX24
Protein Accession Number:
O75426
Ncbi Gene Id Number:
26261
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the F-box protein familywhich is characterized by an approximately 40 amino acid motif, theF-box. The F-box proteins constitute one of the four subunits ofthe ubiquitin protein ligase complex called SCFs(SKP1-cullin-F-box), which function in phosphorylation-dependentubiquitination. The F-box proteins are divided into 3 classes: Fbwscontaining WD-40 domains, Fbls containing leucine-rich repeats, andFbxs containing either different protein-protein interactionmodules or no recognizable motifs. The protein encoded by this genebelongs to the Fbxs class. Multiple transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Background Reference 1:
Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)
Background Reference 2:
Cenciarelli, C., et al. Curr. Biol. 9(20):1177-1179(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-556

List Size:
400 ul

List Price:

$495.00

Datasheet
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