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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

FBXO32 Antibody

CATALOG NUMBER: 61-173

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This FBXO32 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-49 amino acids from human FBXO32.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
IgG1
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXO32
Additional Names:
F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein, MAFbx, FBXO32
Protein Accession Number:
Q969P5
Protein Gi Number:
20177894
Ncbi Gene Id Number:
114907
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Background Reference 1:
Bodine S.C.,et al.Science 294:1704-1708(2001).
Background Reference 2:
Sivertsen E.A.,et al.Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
Background Reference 3:
Pescatori M.,et al.Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
Background Reference 4:
Ota T.,et al.Nat. Genet. 36:40-45(2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-173

List Size:
200 ul

List Price:

$495.00

Datasheet
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