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Western blot analysis in CHO cell line lysates (35ug/lane).
FBXO34 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in CHO cell line lysates (35ug/lane).

FBXO34 Antibody

CATALOG NUMBER: 62-010

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Hamster, Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This FBXO34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 600-629 amino acids from the C-terminal region of human FBXO34.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
79 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXO34
Additional Names:
F-box only protein 34, FBXO34, FBX34
Protein Accession Number:
Q9NWN3
Protein Gi Number:
61252689
Ncbi Gene Id Number:
55030
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Members of the F-box protein family, such as FBXO34, arecharacterized by an approximately 40-amino acid F-box motif. SCFcomplexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM603134), and F-box proteins, act as protein-ubiquitin ligases.F-box proteins interact with SKP1 through the F box, and theyinteract with ubiquitination targets through other proteininteraction domains (Jin et al., 2004 [PubMed 15520277]).[suppliedby OMIM].
Background Reference 1:
Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
Background Reference 2:
Jin, J., et al. Genes Dev. 18(21):2573-2580(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-010

List Size:
400 ul

List Price:

$495.00

Datasheet
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