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45-583 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-583 (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
45-583 staining (0.3ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

FBXW2 Antibody

CATALOG NUMBER: 45-583

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KRGSSFLAGEHPG
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5ug/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both bands were successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3ug/ml. An anonymous customer found positive results in IHC on Mouse kidney tissue. An anonymous customer found positive results in IHC on Rat kidney tissue.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FBXW2
Additional Names:
FBXW2, F-box and WD-40 domain protein 2, FBW2, Fwd2, MGC117371, Md6, F-box protein Fbw2, F-box and WD repeat domain containing 2
Protein Accession Number:
NP_036296.2
Protein Gi Number:
6912360
Ncbi Gene Id Number:
26190
Background Reference 1:
Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW. A family of mammalian F-box proteins. Curr Biol. 1999 Oct 21;9(20):1180-2.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-583

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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