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Western blot analysis in Hela cell line lysates (35ug/lane).
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
FBXW8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

FBXW8 Antibody

CATALOG NUMBER: 55-367

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This FBXW8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-299 amino acids from the Central region of human FBXW8.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
67 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBXW8
Additional Names:
F-box/WD repeat-containing protein 8, F-box and WD-40 domain-containing protein 8, F-box only protein 29, FBXW8, FBW6, FBW8, FBX29, FBXO29, FBXW6
Protein Accession Number:
Q8N3Y1
Protein Gi Number:
296434513
Ncbi Gene Id Number:
26259
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the F-box protein family,members of which are characterized by an approximately 40 aminoacid motif, the F-box. The F-box proteins constitute one of thefour subunits of ubiquitin protein ligase complex called SCFs(SKP1-cullin-F-box), which function in phosphorylation-dependentubiquitination. The F-box proteins are divided into three classes:Fbws containing WD-40 domains, Fbls containing leucine-richrepeats, and Fbxs containing either different protein-proteininteraction modules or no recognizable motifs. The protein encodedby this gene contains a WD-40 domain, in addition to an F-boxmotif, so it belongs to the Fbw class. Alternatively splicedtranscript variants encoding distinct isoforms have been identifiedfor this gene.
Background Reference 1:
Tsutsumi, T., et al. Mol. Cell. Biol. 28(2):743-751(2008)
Background Reference 2:
Koch, H.B., et al. Cell Cycle 6(2):205-217(2007)
Background Reference 3:
Okabe, H., et al. PLoS ONE 1, E128 (2006) :
Background Reference 4:
Watanabe, N., et al. Proc. Natl. Acad. Sci. U.S.A. 101(13):4419-4424(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-367

List Size:
400 ul

List Price:

$495.00

Datasheet
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