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43-625 (0.3ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-625 (0.3ug/ml) staining of Human Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

FCER1A Antibody

CATALOG NUMBER: 43-625

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-TGKVWQLDYESEP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Pripheral Blood Mononucleocytes lysates (calculated MW of 29.6kDa according to NP_001992.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ganguly et al, J Biol Chem. 2007 Nov 9;282(45):32758-64.PMID: 17728245). Recommended concentration: 0.3-1ug/ml.
Predicted Molecular Weight:
Approx 50kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FCER1A
Additional Names:
FCER1A, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, FCE1A, FcERI, Fc IgE receptor, alpha polypeptide, Fc epsilon RI alpha-chain, Fc-epsilon RI-alpha, high affinity immunoglobulin epsilon receptor alpha-subunit, high affinity immunoglobulin epsilon receptor subunit alpha, igE Fc receptor subunit alpha, immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide
Protein Accession Number:
NP_001992.1
Protein Gi Number:
119865
Ncbi Gene Id Number:
2205
Background Reference 1:
Vasudev M, Cheung DS, Pincsak H, Li SH, Yan K, Simpson P, Dasu T, Grayson MH.Expression of high-affinity IgE receptor on human peripheral blood dendritic cells in children.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-625

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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