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Western blot analysis of FCGR1B Antibody in CEM cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human tonsil reacted with FCGR1B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of FCGR1B Antibody in CEM cell line lysates (35ug/lane)

FCGR1B Antibody

CATALOG NUMBER: 63-832

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FCGR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the C-terminal region of human FCGR1B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FCGR1B
Additional Names:
High affinity immunoglobulin gamma Fc receptor IB, IgG Fc receptor IB, Fc-gamma RIB, FcRIB, hFcgammaRIB, FCGR1B, IGFRB
Protein Accession Number:
Q92637
Protein Gi Number:
74760649
Ncbi Gene Id Number:
2210
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FCGR1B may bind to the Fc region of immunoglobulins gamma with a low affinity compared to FCGR1A. This protein may function in the humoral immune response.
Background Reference 1:
Thomas,G., et.al., Nat. Genet. 41 (5), 579-584 (2009)
Background Reference 2:
Kuwano,Y., et.al., Arch. Dermatol. Res. 298 (10), 493-498 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-832

List Size:
400 ul

List Price:

$495.00

Datasheet
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