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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis of FCGR2A using rabbit polyclonal FCGR2A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FCGR2A gene.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

FCGR2A Antibody

CATALOG NUMBER: 57-224

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FCGR2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the C-terminal region of human FCGR2A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FCGR2A
Additional Names:
Low affinity immunoglobulin gamma Fc region receptor II-a, IgG Fc receptor II-a, CDw32, Fc-gamma RII-a, Fc-gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2
Protein Accession Number:
P12318
Protein Gi Number:
160332371
Ncbi Gene Id Number:
2212
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one member of a family of immunoglobulinFc receptor genes found on the surface of many immune responsecells. The protein encoded by this gene is a cell surface receptorfound on phagocytic cells such as macrophages and neutrophils, andis involved in the process of phagocytosis and clearing of immunecomplexes. Alternative splicing results in multiple transcriptvariants.
Background Reference 1:
Dornan, D., et al. Blood 116(20):4212-4222(2010)
Background Reference 2:
Zhang, C.Y., et al. J. Biol. Chem. 285(44):34250-34258(2010)
Background Reference 3:
Iwasaki, M., et al. Breast Cancer Res. Treat. (2010) In press :
Background Reference 4:
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-224

List Size:
400 ul

List Price:

$495.00

Datasheet
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