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Western blot analysis of FCHO2 in rat heart tissue lysate with FCHO2 antibody at (A) 1 and (B) 2 μg/ml
Immunohistochemistry of FCHO2 in human heart tissue with FCHO2 antibody at 2.5 μg/mL.
Immunofluorescence of FCHO2 in human heart tissue with FCHO2 antibody at 20 μg/mL.
Western blot analysis of FCHO2 in rat heart tissue lysate with FCHO2 antibody at (A) 1 and (B) 2 μg/ml
Trial Size

FCHO2 Antibody

CATALOG NUMBER: 6871

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
FCHO2 antibody was raised against a 15 amino acid synthetic peptide near the center of human FCHO2.
The immunogen is located within amino acids 340 - 390 of FCHO2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
FCHO2 antibody can be used for detection of FCHO2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
FCHO2 antibody is predicted to not cross-react with other FCHO protein family members. At least the isoforms of FCHO2 are known to exist; this antibody will detect only the two longest isoforms.
Positive Control 1:
Cat. No. 1461 - Rat Heart Tissue Lysate
Positive Control 2:
Cat. No. 10-501 - Human Heart Tissue Slide
Purification:
FCHO2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FCHO2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FCHO2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FCHO2
Additional Names:
FCHO2 Antibody: FCH domain only protein 2
Protein Accession Number:
NP_620137
Protein Gi Number:
226371723
Ncbi Gene Id Number:
115548
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FCHO2 Antibody: FCHO2 (FCH domain only 2) is a ubiquitously expressed member of the FCFBS superfamily characterized by FES-CIP4 homology (FCH) domain, an FBH domain, and an SH3 domain. Both FCHO2 and the related protein FCHO1 are mediators of clathrin-mediated endocytosis, acting to sculpt the initial bud site and recruit the clathrin machinery for clathrin-coated vesicle formation. FCHO2 binds to Eps15, an important adaptor protein in clathrin-mediated endocytosis.
Background Reference 1:
Katoh M and Katoh M. Identification and characterization of human FCHO2 and mouse fcho2 genes in silico. Int. J. Mol. Med. 2004; 14:327-31
Background Reference 2:
Henne WM, Boucrot E, Meinecke M, et al. FCHo proteins are nucleators of clathrin-mediated endocytosis. Science 2010; 328:1281-4.
Background Reference 3:
Uezu A, Umeda K, Tsujita K, et al. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells 2011; 16:868-78.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6871

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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