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Antibody is used in Western blot to detect FER in HL-60 cell lysate.
FER Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of FER Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Antibody is used in Western blot to detect FER in HL-60 cell lysate.

FER Antibody

CATALOG NUMBER: 63-159

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 782-811 amino acids from the C-terminal region of human FER.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
95 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FER
Additional Names:
Tyrosine-protein kinase Fer, Feline encephalitis virus-related kinase FER, Fujinami poultry sarcoma/Feline sarcoma-related protein Fer, Proto-oncogene c-Fer, Tyrosine kinase 3, p94-Fer, FER, TYK3
Protein Accession Number:
P16591
Protein Gi Number:
97536202
Ncbi Gene Id Number:
2241
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FER is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors.
Background Reference 1:
Greer, P., Nat. Rev. Mol. Cell Biol. 3(4):278-289 (2002).
Background Reference 2:
Rosato, R., et al., Mol. Cell. Biol. 18(10):5762-5770 (1998).
Background Reference 3:
Kim, L., et al., J. Biol. Chem. 273(36):23542-23548 (1998).
Background Reference 4:
Warrington, J.A., et al., Genomics 11(3):701-708 (1991).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-159

List Size:
400 ul

List Price:

$495.00

Datasheet
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