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Western blot analysis using FES monoclonal antibody against truncated FES - His recombinant protein.
Immunofluorescense staining of methanol - fixed Jurkat (top) and Raji (bottom) cells.
Western blot analysis using FES monoclonal antibody against truncated FES - His recombinant protein.

FES Antibody [5A11G5]

CATALOG NUMBER: 32-153

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant FES-His expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
FES monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FES
Additional Names:
FPS, Proto-oncogene c-FesFPS
Protein Accession Number:
P07332
Protein Gi Number:
115502390
Ncbi Gene Id Number:
2242
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FES (feline sarcoma oncogene) and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases.FES and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains. FES was originally identified as an oncogene from avian and feline retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Mutations may activate the FES kinase and thereby contribute to cancer. However, recent data strongly suggests that the c-FES protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer.
Background Reference 1:
Delfino FJ. Stevenson H. Smithgall TE. J Biol Chem. 2006, Mar 31, 281(13): 8829-35. Epub 2006 Feb 2.
Background Reference 2:
Durfee WK. Rivard A. J Biomech Eng. 2005, Nov, 127(6):1014-9.
Background Reference 3:
Vitenzon AS. Mironov EM. Petrushanskaya KA. Neurosci Behav Physiol. 2005, Sep, 35(7):709-14.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-153

List Size:
0.1 mL

List Price:

$432.00

Datasheet
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