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Western blot analysis of FFAR3 Antibody in Jurkat cell line lysates (35ug/lane)
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of FFAR3 Antibody in Jurkat cell line lysates (35ug/lane)

FFAR3 Antibody

CATALOG NUMBER: 61-840

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FFAR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from the C-terminal region of human FFAR3.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FFAR3
Additional Names:
Free fatty acid receptor 3, G-protein coupled receptor 41, FFAR3, GPR41
Protein Accession Number:
O14843
Ncbi Gene Id Number:
2865
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FFAR3 is receptor for short chain fatty acids. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation.
Background Reference 1:
Liaw, C.W., et al. DNA Cell Biol. 28(11):555-560(2009)
Background Reference 2:
Tazoe, H., et al. Biomed. Res. 30(3):149-156(2009)
Background Reference 3:
Stoddart, L.A., et al. J. Biol. Chem. 283(47):32913-32924(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-840

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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