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Western blot analysis of extracts of various cell lines, using FGD1 antibody (16-578) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat kidney using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FGD1 antibody (16-578) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using FGD1 Polyclonal Antibody (16-578) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using FGD1 antibody (16-578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

FGD1 Antibody

CATALOG NUMBER: 16-578

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
U-87MG
Positive Control 2:
BxPC-3
Positive Control 3:
HepG2
Positive Control 4:
U2OS
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FGD1
Additional Names:
FGD1, AAS, FGDY, MRXS16, ZFYVE3
Ncbi Gene Id Number:
2245
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of faciogenital dysplasia and X-linked mental retardation, syndromatic 16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-578

List Size:
100 uL

List Price:

$436.00

Datasheet
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