FGF-17 Antibody

Application Note:

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-17 (1.5 ng/mL), a concentration of 0.02 - 0.035 μg/mL of this antibody is required.
ELISA:
To detect hFGF-17 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-17.
Sandwich: To detect hFGF-17 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-17 (XP-5133Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17.
Western Blot:
To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.