FGF-4 Antibody (biotin)

Application Note:

ELISA:
Sandwich:
To detect hFGF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human FGF-4 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4.
Western Blot:
To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.