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FGF1 Recombinant Protein

CATALOG NUMBER: 91-033

Specifications
source-species:
E. coli
Species:
Mouse
source-species:
E. coli
Recombinant Protein Sequence:
Phe16-Asp155
Fusion Tag:
Tag Free
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Predicted Molecular Weight:
15.7 kD
Biological Activity:
ED50 is less than 0.5 ng/ml as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of Heparin.
Specific Activity of 2.0 x 106 IU/mg.
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 500mM NaCl, pH 6.6. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Storage Conditions:
Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at -20°C for 3 months.
Ncbi Official Symbol:
Fgf1
Additional Names:
Fibroblast Growth Factor 1, FGF-1, Acidic Fibroblast Growth Factor, aFGF, Heparin-Binding Growth Factor 1, HBGF-1, Fgf1, Fgfa, FGF acidic
Protein Accession Number:
P61148
Ncbi Gene Id Number:
14164
Background:
FGF acidic is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

91-033

List Size:
0.05 mg

List Price:

$328.00

Datasheet
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