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43-179 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-179 (1:5000) immunostaining of FGF14-positive axon initial segments in cryosection of an immersion-fixed (1% PFA/0.5% methanol) human hypothalamus*. Antigen retrieval with citrate buffer pH 6 at 80C for 30min. HRP-staining with Ni-DAB, after Biotin-SP
43-179 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Fgf14 Antibody

CATALOG NUMBER: 43-179

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: REQHWDRPSASR-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in Mouse and Human Brain lysates (calculated MW of 28.3kDa according to NP_997550.1 and 27.7kDa according to NP_034331.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Cryosection of immersion-fixed (1% PFA/0.5% methanol) Human Hypothalamus. Optimal dilution: 1:5000. *Vibratome prepared sample for optimal labelling.
Specificity:
This antibody is expected to recognize mouse isoform a (NP_034331.2) and human isoform 1A (NP_004106.1).
Predicted Molecular Weight:
Approx 28kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Fgf14
Additional Names:
Fgf14, fibroblast growth factor 14, FHF-4, Fhf4, mFHF-4(1B), FGF-14, fibroblast growth factor homologous factor 4
Protein Accession Number:
NP_034331.2,NP_997550.1
Protein Gi Number:
46575905
Ncbi Gene Id Number:
2259
Background Reference 1:
Shakkottai VG, Xiao M, Xu L, Wong M, Nerbonne JM, Ornitz DM, Yamada KA.FGF14 regulates the intrinsic excitability of cerebellar Purkinje neurons.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-179

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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