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45-588 (0.3ug/ml) staining of Human Brain ((Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-588 (0.3ug/ml) staining of Human Brain ((Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

FGF23 Antibody

CATALOG NUMBER: 45-588

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RHTRSAEDDSERD
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28+37kDa bands observed in Human Brain (Hippocampus) lysates (calculated MW of 27.9kDa according to NP_065689.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Garringer et al, Am J Physiol Endocrinol Metab. 2008 Oct;295(4):E929-37. PMID: 18682534). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour.
Positive Control 1:
Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FGF23
Additional Names:
FGF23, fibroblast growth factor 23 , ADHR, HPDR2, HYPF, PHPTC , tumor-derived hypophosphatemia inducing factor
Protein Accession Number:
NP_065689.1
Protein Gi Number:
10190674
Ncbi Gene Id Number:
8074
Background Reference 1:
Saito H, Kusano K, Kinosaki M, Ito H, Hirata M, Segawa H, Miyamoto K, Fukushima N. Human fibroblast growth factor-23 mutants suppress Na+-dependent phosphate co-transport activity and 1alpha, 25-dihydroxyvitamin D3 production. J Biol Chem. 2003 Jan 24;278(4):2206-11. Epub 2002 Nov 4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-588

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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