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Western blot analysis of FGF4 in NIH 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.
Immunofluorescence of FGF4 in 3T3 cells with FGF4 antibody at 2.5 μg/mL.
Western blot analysis of FGF4 in NIH 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

FGF4 Antibody

CATALOG NUMBER: 4433

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Chicken: (88%)
Immunogen:
FGF4 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4.
The immunogen is located within the last 50 amino acids of FGF4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
FGF4 antibody can be used for detection of FGF4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
FGF4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FGF4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FGF4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FGF4
Additional Names:
FGF4 Antibody: HST, KFGF, HST-1, HSTF1, K-FGF, HBGF-4, HST, KS3, Fibroblast growth factor 4, Heparin secretory-transforming protein 1, FGF-4
Protein Accession Number:
P08620
Protein Gi Number:
122750
Ncbi Gene Id Number:
2249
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FGF4 Antibody: Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
Background Reference 1:
Powers CJ, McLeskey SW, and Wellstein A. Fibroblast growth factors, their receptors and signaling. Endocr. Relat. Cancer2000; 7:165-97.
Background Reference 2:
Delli-Bovi P, Curatola AM, Kern FG, et al. An oncogene isolated by transfection of Kaposi's sarcoma DNA encodes a growth factor that is a member of the FGF family. Cell1987; 50:729-37.
Background Reference 3:
Yoshida T, Ishimaru K, Sakamoto H, et al. Angiogenic activity of the recombinant hst-1 protein. Cancer Lett.1994; 83:261-268.
Background Reference 4:
Laufer E, Nelson CE, Johnson RL, et al. Sonic hedgehog and Fgf-4 act through a signaling cascade and feedback loop to integrate growth and patterning of the developing limb bud. Cell1994; 79:993-1003.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4433

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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