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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Western blot analysis of FGF9 using rabbit polyclonal FGF9 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FGF9 gene.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).

FGF9 Antibody

CATALOG NUMBER: 55-089

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Rat
Immunogen:
This FGF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-58 amino acids from the N-terminal region of human FGF9.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
23 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FGF9
Additional Names:
Fibroblast growth factor 9, FGF-9, Glia-activating factor, GAF, Heparin-binding growth factor 9, HBGF-9, FGF9
Protein Accession Number:
P31371
Protein Gi Number:
544290
Ncbi Gene Id Number:
2254
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thefibroblast growth factor (FGF) family. FGF family members possessbroad mitogenic and cell survival activities, and are involved in avariety of biological processes, including embryonic development,cell growth, morphogenesis, tissue repair, tumor growth andinvasion. This protein was isolated as a secreted factor thatexhibits a growth-stimulating effect on cultured glial cells. Innervous system, this protein is produced mainly by neurons and maybe important for glial cell development. Expression of the mousehomolog of this gene was found to be dependent on Sonic hedgehog(Shh) signaling. Mice lacking the homolog gene displayed amale-to-female sex reversal phenotype, which suggested a role intesticular embryogenesis.
Background Reference 1:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 2:
Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010) :
Background Reference 3:
Kalinina, J., et al. Mol. Cell. Biol. 29(17):4663-4678(2009)
Background Reference 4:
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-089

List Size:
400 ul

List Price:

$495.00

Datasheet
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