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45-589 (0.3ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-589 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-589 (0.3ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

FGFR1 Antibody

CATALOG NUMBER: 45-589

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: CLPRHPAQLANGGLKR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in Human Breast lysates (calculated MW of 82.2kDa according to NP_075593, 81.9kDa according to NP_075594). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of the enterocytic cytoplasm. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 4ug/ml.
Specificity:
This antibody is expected to recognise five isoforms (as represented by NP_075598.2; NP_056934.2; NP_075593.1; NP_075594.1; NP_075599.1).
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FGFR1
Additional Names:
FGFR1, H2, H3, H4, H5, CEK, FLG, FLT2, KAL2, BFGFR, C-FGR, N-SAM, fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome), FLG protein, N-sam tyrosine kinase, tyrosylprotein kinase, protein-tyrosine kinase, FMS-like tyrosine kinase 2, hydroxyaryl-protein kinase, fms-related tyrosine kinase-2, heparin-binding growth factor receptor, basic fibroblast growth factor receptor 1, fibroblast growth factor receptor 1, CD331, FGFBR, FLJ99988, HBGFR, OGD, OTTHUMP00000190881, soluble FGFR1 variant 1, soluble FGFR1 variant 2
Protein Accession Number:
NP_075598.2,NP_056934.2,NP_075593.1,NP_075594.1,NP_075599.1
Protein Gi Number:
11276087
Ncbi Gene Id Number:
2260
Background Reference 1:
Torry DS, Mukherjea D, Arroyo J, Torry RJ. Expression and function of placenta growth factor: implications for abnormal placentation. J Soc Gynecol Investig. 2003 May;10(4):178-88. Review.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-589

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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