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Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 μg/ml.
Immunofluorescence of FHIT in HeLa cells with FHIT antibody at 5 μg/mL.
Western blot analysis of FHIT in HeLa cell lysate with FHIT antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

FHIT Antibody

CATALOG NUMBER: 7455

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%), Rat: (72%)
Immunogen:
FHIT antibody was raised against an 18 amino acid peptide near the carboxy terminus of human FHIT.
The immunogen is located within the last 50 amino acids of FHIT .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
FHIT antibody can be used for detection of FHIT by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
FHIT antibody is human and mouse reactive.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 16 kDa
Observed: 15kDa
Purification:
FHIT Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FHIT Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FHIT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
FHIT
Additional Names:
FHIT Antibody : FRA3B, AP3AaseAP3A hydrolase, AP3Aase
Protein Accession Number:
NP_002003
Protein Gi Number:
4503719
Ncbi Gene Id Number:
2272
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FHIT is member of the histidine triad gene family and is a diadenosine involved in purine metabolism (1). FHIT is also thought to be a tumor suppressor gene and is involved in multiple apoptotic pathways (1,2). The FHIT gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene (3). Aberrant transcripts from this gene have been found in multiple carcinomas (4).
Background Reference 1:
Barnes LD, Garrison PN, Siprashvili Z, et al. Fhit, a putative tumor suppressor in humans, is a dinucleotide 5',5'''-P1,P3-triphosphate hydrolase. Biochemistry 1996; 35:11529-35.
Background Reference 2:
Wali A. FHIT: doubts are clear now. ScientificWorldJournal 2010; 10:1142-51.
Background Reference 3:
Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell 1996; 84:587-97.
Background Reference 4:
Drusco A, Pekarsky Y, Costinean S, et al. Common fragile site tumor suppressor genes and corresponding mouse models of cancer. J. Biomed. Biotechnol.2011; Epub 2010 Dec 29.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7455

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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