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45-592 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-592 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

FHL2 Antibody

CATALOG NUMBER: 45-592

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: RDDILCPDCGKDI
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 30kDa band observed in Human and Mouse Heart lysates (calculated MW of 32.2kDa according to NP_001441.4). Recommended concentration: 1-3ug/ml.
Specificity:
Reported variants represent identical protein (NP_001441.4; NP_963849.1; NP_963851.2; NP_001034581.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FHL2
Additional Names:
FHL2, DRAL, AAG11, SLIM3, four and a half LIM domains 2, down-regulated in rhabdomyosarcoma LIM protein, aging-associated gene 11, four and a half LIM-domain protein 2, OTTHUMP00000203164, OTTHUMP00000203168, OTTHUMP00000203169
Protein Accession Number:
NP_001441.4
Protein Gi Number:
87159832
Ncbi Gene Id Number:
2274
Background Reference 1:
Morgan MJ, Madgwick AJ. Slim defines a novel family of LIM-proteins expressed in skeletal muscle. Biochem Biophys Res Commun. 1996 Aug 14;225(2):632-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-592

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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