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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with FHL5 using FHL5 Rabbit mAb at 1:4000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5 s.
Western blot analysis of lysates from Mouse heart using FHL5 Rabbit mAb at 1:4000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45 s.
Confocal imaging of 293T cells transfected with FHL5 using FHL5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunoprecipitation of FHL5 in 300 µg extracts from 293T cells transfected with FHL5, using 3 µg FHL5 Rabbit mAb. Western blot analysis was performed using FHL5 Rabbit mAb at 1:3000 dilution.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with FHL5 using FHL5 Rabbit mAb at 1:4000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5 s.

FHL5 Rabbit Recombinant Antibody (83-054)

CATALOG NUMBER: 83-054

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IP, WB
Application Note:
WB,1:1000 - 1:5000, IF/ICC,1:50 - 1:200, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T transfected with FHL5
Predicted Molecular Weight:
33kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FHL5
Additional Names:
ACT; FHL-5; dJ393D12.2; 1700027G07Rik; FHL5
Protein Accession Number:
Q5TD97
Ncbi Gene Id Number:
9457
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Polymorphisms in this gene may be associated with susceptibility for migraine headaches. Alternative splicing results in multiple transcript variants encoding the same protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-054

List Size:
100 uL

List Price:

$477.00

Datasheet
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