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Western blot analysis of extract from 293, HeLa cells using Fibrillarin Antibody.
Western blot analysis of extract from 293, HeLa cells using Fibrillarin Antibody.

Fibrillarin Antibody

CATALOG NUMBER: 79-685

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fibrillarin antibody was raised against a peptide sequence around aa.297~301 ( L-T-L-E-P) derived from Human Fibrillarin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total Fibrillarin protein.
Predicted Molecular Weight:
37 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
FBL
Additional Names:
FIB, FLRN, RNU3IP1, FBL, FIB
Protein Accession Number:
NP_001427.2
Protein Gi Number:
12056465
Ncbi Gene Id Number:
2091
Background:
Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.
Background Reference 1:
Lischwe M. A., Ochs R. L., Reddy R., Cook R. G., Yeoman L. C., Tan E. M., Reichlin M., Busch H. J. Biol. Chem. 260:14304-14310 (1985) Yanagida M., Hayano T., Yamauchi Y., Shinkawa T., Natsume T., Isobe T., Takahashi N. J. Biol. Chem. 279:1607-1614 (2004) Wang Y., Liu J., Zhao H., Lue W., Zhao J., Yang L., Li N., Du X., Ke Y. Biochim. Biophys. Acta 1773:863-868 (2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-685

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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