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Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.
Immunofluorescence of Fibulin 3 in HeLa cells with Fibulin 3 antibody at 20 μg/mL.
Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

Fibulin 3 Antibody

CATALOG NUMBER: 5213

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fibulin 3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human Fibulin 3.
The immunogen is located within amino acids 410 - 460 of Fibulin 3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Fibulin 3 antibody can be used for detection of Fibulin 3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 52 kDa
Observed: 47 kDa
Purification:
Fibulin 3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Fibulin 3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Fibulin 3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EFEMP1
Additional Names:
Fibulin 3 Antibody: DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, FBLN3, FIBL-3, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5
Protein Accession Number:
NP_001034437
Protein Gi Number:
9665262
Ncbi Gene Id Number:
2202
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fibulin 3 Antibody: Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target.
Background Reference 1:
Kobayashi N, Kostka G, Garbe JH, et al. A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization. J. Biol. Chem. 2007; 282:11805-16.
Background Reference 2:
Marmorstein LY, Munier FL, Arsenijevic Y, et al. Aberrant accumulation of EFEMP1 underlies drusen formation in Malattia Leventinese and age-related macular degeneration. Proc. Natl. Acad. Sci. USA 2002; 99:13067-72.
Background Reference 3:
McLaughlin PJ, Bakall B, Choi J, et al. Lack of fibulin-3 causes early aging and herniation, but not macular degeneration in mice. Hum. Mol. Genet. 2007; 16:3059-70.
Background Reference 4:
Seeliger H, Camaj P, Ischenko I, et al. EFEMP1 expression promotes in vivo tumor growth in human pancreatic adenocarcinoma. Mol. Cancer Res. 2009; 7:189-98.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5213

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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